Complementary Proteomics – Breaking the Knowledge Barrier
Complementary proteomics – the integration of different high-performance techniques – uses multiple platforms to resolve a biological question or puzzle. Each of those technologies unlocks a piece of the answer. Viewed as a whole, they provide a multi-dimensional analysis unavailable when viewed as individual analytical methods.
Here are three key areas where different mass spec technologies can collectively help break the proteomics knowledge barrier and solve more complex proteomics questions:
- BioMarker Discovery & Validation
For biomarker discovery and validation, UHR-QTOF is generally considered an exceptionally powerful and easy-to-use technique. In the case of Bruker’s UHR-QTOF – the impact HD – our system is especially powerful when it comes to analyzing complex mixtures with short (60-90 minute) gradients.Learn more about the impact HD, which offers an array of new industry benchmarks – from ease of use (zero adjustment) to performance stability, to power (5X magnitude intra-spectral dynamic range).
- Glycopeptide Characterization
For unrivaled glycopeptide sensitivity and characterization, QTOF (learn more about Bruker’s QTOF captiveSpray nanoBooster technology), delivers unique characterization capabilities and glycoproteomics workflows that enable the screening and characterization of glycans and glycopeptides. QTOF can deliver certainty in PTM characterization with MS/MS resolution and accuracy at speed, together with fantastic intact protein measurement capabilities. Bruker’s amazon ETD, for example, is uniquely suited to labile PTM localization and small protein top-down analysis capabilities. Ideally, a high-end QTOF system will be complemented with MALDI for glycopeptide screening and ISD top-down proteomics capabilities.
- Imaging – the Spatial Frontier
A third key element to solving complex proteomics challenges – spatial information (in this case, tissue distribution) – isn’t available via the two other approaches, above. Tissue distribution data can be obtained using MALDI imaging workflows, which support both intact protein (top-down profiling) and image ID (Bottom-up imaging) techniques. (By the way, when it comes to system flexibility, we consider our MALDI FLEX series to be the hands-down champion, capable of on-tissue biomarker discovery as well as full glycopeptide analysis or high-speed, wide mass range Top-Down analyses.)
Information on the identification, regulation, characterization and distribution of proteins is critical to deciphering complex biological questions. A complementary approach to proteomics in which different techniques are applied delivers multi-faceted data that provides a more complete understanding of biological function.
What tools does your lab use to answer complex proteomics questions?