Mass Spec – Top-Down Characterization of Glycoproteins
One of our team members – Marcus Macht – over in Bremen, Germany recently published a paper in TrAC (Trends in Analytical Chemistry) highlighting success with top-down characterization using Bruker’s mass spectrometry tools.
Mass spec has traditionally served as a bottom-up technique – looking at the peptides derived from chemical or enzymatic digestion of the protein, rather than the protein itself.
Using electrospray ionization, matrix-assisted laser desorption/ionization (MALDI) and top-down fragmentation techniques, however, allows the characterization of an intact (glyco)protein – not just its peptide building blocks.
The advantage? Top-down analysis ensures coverage of the whole protein sequence during characterization. It’s a new way of using mass spec to perform protein characterization, delivering critical data and opening up entirely new avenues of research.